Journal: PLoS ONE

Article Title: Rectified Cell Migration on Saw-Like Micro-Elastically Patterned Hydrogels with Asymmetric Gradient Ratchet Teeth

doi: 10.1371/journal.pone.0078067

Figure Lengend Snippet: The conditions used to prepare the gels are described in . (a) Phase contrast microscopy image of the gel. Sale bar: 100 µm. (b) Distribution of Young’s modulus in a single unit of the gel. Unit size: 90 µm. Elasticity peak: ca. 500 kPa. (c) Trajectories of 3T3 fibroblasts cultured on gels with PSLs. Cells were observed by time-lapse microscopy for 24 hr and images were acquired at 15 min intervals. (d) Time-course of ensemble averaged trajectories with standard deviation in the presence (red) and absence (blue) of PSLs. n=30 cells.

Article Snippet: The mouse fibroblast cell line (NIH/3T3) was purchased from Dainippon Sumitomo Pharmaceutical Co. Ltd. (Osaka, Japan) and cultured in Dulbecco’s modified Eagle’s medium (Gibco BRL, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Gibco BRL), 3.5 g/l glucose, 2 mM L-glutamine, 50 units/ml penicillin, and 50 μg/ml streptomycin.

Techniques: Microscopy, Cell Culture, Time-lapse Microscopy, Standard Deviation

Journal: Transactions of the American Ophthalmological Society

Article Title: Stimulating Retinal Blood Vessel Protection with Hypoxia-Inducible Factor Stabilization: Identification of Novel Small-Molecule Hydrazones to Inhibit Hypoxia-Inducible Factor Prolyl Hydroxylase (An American Ophthalmological Society Thesis)

doi:

Figure Lengend Snippet: High-throughput reporter assay at 10 μg/mL used to identify small molecules that inhibit hypoxia-inducible factor prolyl hydroxylase (HIF PHD). Twenty-three new small molecules were discovered by the high-throughput assay using NIH3T3 cell line stably expressing the reporter gene of firefly luciferase under a HIF-1-inducible promoter (NIH3T3/HIF-luc; Panomics, Santa Clara, California). See Appendix for a list of the chemical names of these 23 compounds in the same order as in this Figure.

Article Snippet: Screening for compounds that induce HIF-1 activity was performed using NIH3T3 cell line stably expressing the reporter gene of firefly luciferase under a HIF-1-inducible promoter (NIH3T3/HIF-luc, Panomics, Santa Clara, California).

Techniques: High Throughput Screening Assay, Reporter Assay, Stable Transfection, Expressing, Luciferase